MIRA Plant Transgenic NOS Gene Fluorescence Detection Kit

MIRA Plant Transgenic NOS Gene Fluorescence Detection Kit

This kit is based on a constant temperature isothermal nucleic acid rapid amplification technology: at constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA chain, and the recombinant enzyme, single strand DNA binding protein and DNA polymerase in the reaction system use the newly synthesized cDNA chain as template for rapid nucleic acid amplification reaction.

Product Introduction

【Product name】 MIRA Plant Transgenic CaMV35S Gene Fluorescence Detection Kit

【Packing Specifications】48 T/pack

 

【Background】

 

 

The Cauliflower Mosaic Virus 35S promoter (CaMV35S) is one of the most commonly used regulatory elements in genetically modified (GM) plants. Its presence serves as a universal marker for transgenic crops, making it crucial for GMO screening, regulatory compliance, and transgenic research.

 

【Intended Use】

 

 

The MIRA Plant Transgenic CaMV35S Gene Fluorescence Detection Kit is designed for the rapid and qualitative detection of the CaMV35S gene in plant samples. This kit utilizes MIRA (Multienzyme Isothermal Rapid Amplification) technology to perform sensitive and specific amplification at a constant temperature (39–42°C), combined with real-time fluorescence monitoring for accurate detection. It is intended for research use only and is ideal for GMO testing, plant molecular biology research, and regulatory applications.

 

【Main components】

 

 

Composition

Content

Main ingredient

E buffer

1.0 ml *2 tubes

Reaction buffer

B buffer

150 μL *1tube

Mg2+and so on

Positive control template(003)

100 μL *1tube

synthetic plasmid

Reagents

48T

Enzymes, dNTPs, etc.

 

【Key Features】

 

 

 Rapid detection – Results available within 15–20 minutes, enabling quick screening.
 Isothermal amplification – Simplified workflow without complex PCR equipment.
 High specificity and sensitivity – Reliable identification of CaMV35S gene with minimal cross-reactivity.
 Real-time fluorescence monitoring – Ensures precise and accurate detection.
 Lyophilized reagents – Stable at room temperature, easy to store and transport.
 User-friendly operation – Suitable for laboratory use, field testing, and regulatory inspections.

 

【Application Areas】

 

 

• GMO screening and labeling – Identification of transgenic plants containing the CaMV35S promoter.

• Plant biotechnology research – Verification and monitoring of transgenic events.

• Regulatory compliance – Supporting GMO detection and certification processes.

• Agricultural and environmental studies – Monitoring GM crops in the field.

 

The MIRA Plant Transgenic CaMV35S Gene Fluorescence Detection Kit offers a fast, accurate, and efficient solution for detecting transgenic markers, supporting advancements in plant biotechnology and regulatory oversight.

 

 

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