MIRA Plant Transgenic CaMV35S Gene Fluorescence Detection Kit

This kit is based on a constant temperature isothermal nucleic acid rapid amplification technology: at constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA chain, and the recombinant enzyme, single strand DNA binding protein and DNA polymerase in the reaction system use the newly synthesized cDNA chain as template for rapid nucleic acid amplification reaction.

【Product name】 MIRA Plant transgenic CaMV35S gene Fluorescence Detection Kit

【Packing Specifications】48 T/pack

【Background】

The CaMV35S promoter derived from Cauliflower Mosaic Virus is one of the most widely used regulatory elements in genetically modified (GM) crops. It serves as a key indicator for the identification of transgenic plants. Reliable detection of the CaMV35S gene is essential for GMO screening, regulatory compliance, and transgenic research.

【Intended usage】

The MIRA Plant Transgenic CaMV35S Gene Fluorescence Detection Kit is designed for the rapid, qualitative detection of the CaMV35S gene in plant samples. Utilizing MIRA (Multienzyme Isothermal Rapid Amplification) technology, the kit provides highly sensitive and specific amplification at a constant temperature (39–42°C), with real-time fluorescence detection for accurate monitoring. It is intended for research use only, and ideal for GMO testing, plant biotechnology research, and regulatory inspections.

【Main components】

Composition	Content	Main ingredient
E buffer	1.0 ml *2 tubes	Reaction buffer
B buffer	150 μL *1tube	Mg²⁺and so on
Positive control template(003)	100 μL *1tube	synthetic plasmid
Reagents	48T	Enzymes, dNTPs, etc.

【Key Features】

Rapid detection – Delivers results within 15–20 minutes, ensuring fast decision-making.
Isothermal amplification – Simplifies testing, no need for thermal cycling equipment.
High specificity and sensitivity – Accurate identification of CaMV35S sequences with minimal cross-reactivity.
Real-time fluorescence monitoring – Provides precise amplification tracking and reliable results.
Lyophilized reagents – Stable at room temperature, easy to store and transport.
User-friendly operation – Suitable for laboratories, field testing, and GMO regulatory testing.

【Application Areas】

• GMO screening – Detection of CaMV35S promoter in genetically modified crops.

• Plant biotechnology research – Verification of transgenic plant materials.

• Regulatory compliance testing – Ensuring non-GMO product certification and quality control.

• Agricultural research – Monitoring transgenic plant development and stability.

The MIRA Plant Transgenic CaMV35S Gene Fluorescence Detection Kit offers a fast, reliable, and convenient solution for detecting GMO markers, supporting plant biotechnology advancements and regulatory oversight.

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