MIRA Bursaphelenchus Xylophilus Fluorescence Detection Kit

This kit is based on a rapid nucleic acid isothermal amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands and the recombinase, Single-stranded DNA-binding proteins and DNA polymerases use newly synthesized cDNA strands as templates for rapid nucleic acid amplification reactions.

【Product Name】MIRA Bursaphelenchus xylophilus Fluorescence Detection Kit

【Packing Specifications】48 T/pack

【Background】

Bursaphelenchus xylophilus, commonly known as the pinewood nematode, is the causative agent of pine wilt disease, a devastating forest disease that threatens pine ecosystems worldwide. Early and accurate detection of B. xylophilus is critical for preventing its spread, enabling timely quarantine measures, and protecting forest resources.

【Detection principle】

The MIRA Bursaphelenchus Xylophilus Fluorescence Detection Kit is a rapid, sensitive nucleic acid amplification assay designed for the qualitative detection of pinewood nematode nucleic acid. Using MIRA (Multienzyme Isothermal Rapid Amplification) technology, the kit performs amplification at a constant temperature (39–42°C) and provides real-time fluorescence monitoring, delivering fast and accurate results without the need for thermal cycles. It is intended for research use only, ideal for applications in forestry disease management, quarantine inspection, and environmental monitoring.

【Main components】

Composition	Content	Main ingredient
E buffer	1.0 ml *2 tubes	Reaction buffer
B buffer	150 μL *1 tube	Mg²⁺and so on
Positive control template(084)	100 μL *1 tube	synthetic plasmid
Reagents	48T	Enzymes, dNTPs, etc.

【Key Features】

Rapid detection – Provides results within 15–20 minutes, supporting timely decision-making.
Isothermal amplification – Simplifies the process, no need for complex thermal cycling equipment.
High specificity and sensitivity – Accurately identifies B. xylophilus with minimal cross-reactivity.
Real-time fluorescence monitoring – Enables precise amplification tracking and reliable results.
Lyophilized reagents – Stable at room temperature, convenient for storage and transport.
User-friendly workflow – Suitable for laboratories, quarantine stations, and field testing applications.

【Application Areas】

• Forestry disease surveillance – Monitoring and detecting pinewood nematode in affected regions.

• Quarantine inspections – Screening of wood materials and plant products for nematode presence.

• Environmental research – Studying transmission patterns and nematode prevalence.

• Forest management and protection programs – Early detection to implement disease control strategies.

The MIRA Bursaphelenchus xylophilus Fluorescence Detection Kit provides a fast, accurate, and efficient solution for the detection of pinewood nematode, supporting sustainable forest health and effective disease management practices.

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