Fluorescence Detector

Your Leading Fluorescence Detector Supplier

Amp-Future Biotech specializes in the development, production, and sales of isothermal nucleic acid amplification reagents. Our proprietary MIRA technology (Multienzyme Isothermal Rapid Amplification) ensures fast, accurate, and portable molecular detection, suitable for on-site testing in diverse fields including clinical, agriculture, veterinary , food safety and genetic technologies.

Why choose us?

01.

Our certifications

We has obtained certifications such as NMPA, CE, and the Veterinary Drug Registration License etc.

02.

Our factory

The company is equipped with advanced facilities and operates a class 100,000 cleanroom environment. With an annual reagent production capacity exceeding 90 million tests.

03.

Sales market

Our business footprint covers the entire domestic market and extends across Europe, North America, Middle East and Asia.

04.

Custom service

We can undertake independent and joint development of projects, OEM/ODM. Primer probe design, guidance, synthesis and other services.

Types of Detectors

There are two categories: specific and bulk property detectors (2)

01.

Specific HPLC Detectors

Mass spectroscopic detectors – This type of detector provides high sensitivity and selectivity. Analytes are first ionised in the source, these ions are then separated on the basis of their mass/charge ratio before being detected.

UV-VIS HPLC detectors – The types of UV-VIS HPLC detectors are fixed wavelength, variable wavelength, and diode array. They are used specifically for compounds that absorb light.

Photo diode array (PDA) HPLC detectors – The use of multiple diodes allows for the monitoring of more than one absorbing compound at different wavelengths at the same time.

Fluorescence HPLC detectors – This is the most sensitive and specific. Types include single wavelength, multi-wavelength, and laser-induced detectors. This type relies on compounds naturally fluorescing or derivatization to add a fluorescent tag to target analytes allowing for detection to occur.

02.

Bulk Property HPLC Detectors

Electrical conductivity HPLC detectors – These detectors work by measuring mobile phase conductivity using sensors made up of a flow-through cell that contains two electrodes that are made of platinum, steel, or a noble metal.

Refractive index HPLC detectors – Detection relies on changes in the refractive index of the mobile phase due to the presence of eluting compounds RI detectors have the lowest sensitivity but are generally accepted as being universal detectors.

Electrochemical HPLC detectors – This includes equilibrium and dynamic detectors that are sensitive to changes in the composition or flow rate of the mobile phase and sensitive to substances that are reducible or oxidizable.

Light-scattering HPLC detectors – There are two types, including low-angle laser light scattering and multiple-angle laser light scattering detectors. They measure the scattered light, which is ideal for detecting molecules with a high molecular weight as the eluent passes through a sensor cell based on molecular size.

Digital Isothermal Fluorescence Detector

Applications of Fluorescence Detectors

Analytical chemistry: Fluorescence detectors are extensively used in analytical chemistry for the quantification and identification of various compounds. They are highly sensitive and selective, making them suitable for a wide range of applications.

Pharmaceutical analysis: In the pharmaceutical industry, fluorescence detectors are crucial for analyzing drug compounds, including pharmaceuticals, metabolites, and impurities. They are used in quality control and formulation studies.

Environmental monitoring: Fluorescence detectors are employed to detect and measure pollutants, such as polycyclic aromatic hydrocarbons (pahs), heavy metals, and organic contaminants in water, soil, and air samples.

Biomedical research: In biological and biomedical research, fluorescence detectors play a vital role in studying cellular processes, protein interactions, dna sequencing, and gene expression. Fluorescent labels are often used to tag biomolecules for detection.

Clinical diagnostics: Fluorescence detectors are used in clinical laboratories for assays, immunoassays, and tests that rely on fluorescent labels. They are valuable tools for diagnosing diseases and monitoring biomarkers in patient samples.

Food and beverage analysis: Fluorescence detectors are utilized in the food industry to detect and quantify contaminants, additives, vitamins, and other compounds. They are crucial for quality control and safety assessments.

Drug discovery: Fluorescence detectors are employed in high-throughput screening (hts) assays to identify potential drug candidates and evaluate their interactions with specific targets, including receptors and enzymes.

Environmental fluorescence sensors: Fluorescence detectors can be integrated into environmental sensors to continuously monitor specific analytes or parameters, such as ph, oxygen levels, and pollutant concentrations in natural water bodies.

Material science: Fluorescence detectors help researchers investigate the properties and characteristics of materials, such as polymers, nanoparticles, and quantum dots, through fluorescence spectroscopy.

Forensic science: In forensic laboratories, fluorescence detectors are used for the analysis of various substances, including drugs, explosives, and bodily fluids. They can help identify trace amounts of these substances in crime scene investigations.

How does the fluorescence detector work?

The fluorescence detector works by exposing the sample to a beam from a high-energy light source. Depending on the wavelength of light, this will cause certain compounds within the sample to ‘fluoresce’ and produce light of their own at a different wavelength. The detector monitors these emissions and collects data on the specific wavelengths and intensities, which in turn reveals the concentrations of different compounds within the sample. By combining this with the separation caused by HPLC, it is possible to accurately characterize a sample containing fluorescent chemicals.

Parameter

Detector type	Fluorescence detector
Detection channels	1
Number of signals	1
Light source	Xenon lamp
Wavelength range	200 – 650 nm
Spectral bandwidth	20 nm
Wavelength accuracy	± 2 nm
Wavelength precision	± 0.2 nm indicates the precision performance when the power is turned on in the single wavelength mode and the wavelength is changed.
Sensitivity	Can be set at three levels: High (x 1), med (x 32), low (x 1024)
Wetted materials	Sus316l, ptfe (fluorocarbon polymers), quartz
Flow cell volume	12 µl
Time constants	11 levels can be selected, equivalent to “no filter”, 0.05, 0.1, 0.5, 1.0, 1.5, 2.0, 3.0, 6.0, 8.0 and 10.0 seconds
Autozero	Auto zero function, basline shift function

Custom process

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Collaborative Partners

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FAQ

Q: What is a fluorescence detector used for?

A: A fluorescence detector is primarily used in high-performance liquid chromatography (HPLC) applications to detect and measure fluorescent compounds in samples. It is ideal for analyzing trace-level compounds that naturally fluoresce or are tagged with fluorescent markers.

Q: How does a fluorescence detector work?

A: A fluorescence detector works by exposing a sample to specific wavelengths of light, causing fluorescent compounds to emit light at different wavelengths. The detector then captures this emitted light, measuring its intensity to determine the concentration of fluorescent compounds in the sample.

Q: What are the benefits of using a fluorescence detector in HPLC?

A: Fluorescence detectors provide enhanced sensitivity, allowing for the detection of compounds at low concentrations. They also offer high selectivity and low noise, making them suitable for precise, reliable analysis of complex samples.

Q: What kind of compounds can be detected with a fluorescence detector?

A: Fluorescence detectors are ideal for detecting compounds that either naturally fluoresce or can be made fluorescent through tagging, including various organic compounds, proteins, and pharmaceuticals.

Q: Does this fluorescence detector allow for adjustable wavelength settings?

A: Yes, most fluorescence detectors used in HPLC applications allow users to adjust excitation and emission wavelengths to target specific compounds, enhancing flexibility and precision in compound detection.

Q: How does the sensitivity of a fluorescence detector compare to other types of detectors?

A: Fluorescence detectors are generally more sensitive than other types of detectors, such as UV detectors, because they specifically measure compounds that emit fluorescence, resulting in lower detection limits for trace analysis.

Q: Are there specific sample types that are better suited for fluorescence detection?

A: Fluorescence detection is well-suited for samples containing compounds that fluoresce, such as aromatic hydrocarbons, biochemicals like proteins and amino acids, and fluorescent-labeled substances often used in biomedical research and environmental testing.

Q: Is it possible to detect multiple compounds simultaneously with this detector?

A: Yes, with optimized wavelength settings, fluorescence detectors can detect multiple fluorescent compounds in a single run, provided that the compounds have distinct fluorescence properties.

Q: What level of maintenance is required for a fluorescence detector?

A: Fluorescence detectors require regular maintenance, such as lamp replacement and calibration checks, to ensure optimal performance. Maintenance intervals depend on usage and sample types but are typically minimal.

Q: Can this detector be integrated with existing HPLC systems?

A: Yes, fluorescence detectors are generally designed to be compatible with most standard HPLC systems, allowing for easy integration into existing laboratory setups.

We're professional fluorescence detector manufacturers and suppliers in China, specialized in providing high quality customized service. If you're going to buy fluorescence detector at competitive price, welcome to get quotation and free sample from our factory.

fluorescence detector, molecular detection instrument