MIRA Bursaphelenchus Xylophilus Nucleic Acid Test Strip Detection Kit

This kit is based on a rapid nucleic acid isothermal amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands and the recombinase, Single-stranded DNA-binding proteins and DNA polymerases use newly synthesized cDNA strands as templates for rapid nucleic acid amplification reactions.

【Product Name】MIRA Bursaphelenchus xylophilus Nucleic acid test strip

【Packing Specifications】48 T/pack

【Background】

Bursaphelenchus xylophilus, commonly known as the pinewood nematode, is a destructive plant pathogen responsible for pine wilt disease, causing significant ecological and economic damage to forests worldwide. Rapid and accurate detection of B. xylophilus is essential for early intervention, forest protection, and quarantine monitoring.

【Intended usage】

The MIRA Bursaphelenchus xylophilus Nucleic Acid Test Strip Detection Kit is designed for the rapid, qualitative detection of pinewood nematode nucleic acid. Combining MIRA isothermal amplification technology with a lateral flow test strip, the kit provides visual, easy-to-interpret results without the need for specialized laboratory equipment. It is intended for research use only, suitable for applications in forest disease surveillance, quarantine inspections, and on-site pathogen screening.

【Main components】

Composition	Content	Main ingredient
E buffer	1.0 ml *2 tubes	Reaction buffer
B buffer	150 μL *1 tube	Mg²⁺and so on
Positive control template(084)	100 μL *1 tube	synthetic plasmid
Reagents	48T	Enzymes, dNTPs, etc.

【Key Features】

Simple one-step operation – Amplification and result interpretation via test strip in a single process.
Fast detection – Results available within 15–20 minutes, enabling timely forest protection measures.
No complex equipment required – Ideal for field testing and low-resource settings.
Lyophilized reagents – Stable at room temperature, easy to transport and store.
Portable and user-friendly – Suitable for forestry departments, quarantine stations, and environmental monitoring teams.

【Application Areas】

• Forest pest surveillance – Rapid detection of B. xylophilus in affected or at-risk areas.

• Quarantine and customs inspections – On-site screening of wood products and plant materials.

• Environmental research – Study of pine wilt disease transmission and nematode prevalence.

• Field testing and POCT – Immediate, reliable detection without laboratory infrastructure.

The MIRA Bursaphelenchus xylophilus Nucleic Acid Test Strip Detection Kit delivers a fast, portable, and highly effective solution for detecting pinewood nematode, supporting sustainable forest management and disease prevention efforts.

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