MIRA Citrus Huanglongbing Fluorescence Detection Kit

This kit is based on a rapid nucleic acid isothermal amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands and the recombinase, Single-stranded DNA-binding proteins and DNA polymerases use newly synthesized cDNA strands as templates for rapid nucleic acid amplification reactions.

【Product Name】MIRA Citrus Huanglongbing Fluorescence Detection Kit

【Packing Specifications】48 T/pack

【Background】

Citrus Huanglongbing (HLB), also known as citrus greening disease, is a devastating bacterial disease caused by Candidatus Liberibacter species. It severely impacts citrus crops, leading to reduced fruit quality, yield loss, and eventual tree decline. Early detection of HLB is essential for effective disease management, quarantine efforts, and sustaining citrus production worldwide.

【Intended Use】

The MIRA Citrus Huanglongbing Fluorescence Detection Kit is developed for the rapid and sensitive detection of HLB pathogen nucleic acid. Utilizing MIRA (Multienzyme Isothermal Rapid Amplification) technology, the kit enables fast, specific amplification at a constant temperature (39–42°C), combined with real-time fluorescence detection to ensure accurate results. It is intended for research use only, ideal for applications in plant disease research, citrus disease surveillance, and agricultural diagnostics.

【Main components】

Composition	Content	Main ingredient
E buffer	1.0 ml *2 tubes	Reaction buffer
B buffer	150 μL *1 tube	Mg²⁺and so on
Positive control template(084)	100 μL *1 tube	synthetic plasmid
Reagents	48T	Enzymes, dNTPs, etc.

【Key Features】

Rapid detection – Results available within 15–20 minutes, allowing timely intervention.
Isothermal amplification – Simplified workflow without complex thermal cycling instruments.
High specificity and sensitivity – Accurately detects HLB pathogens with minimal cross-reactivity.
Real-time fluorescence monitoring – Enables precise amplification tracking and reliable quantification.
Lyophilized reagents – Stable at room temperature, convenient for storage and transport.
User-friendly operation – Suitable for laboratory testing, field research, and quarantine inspection use.

【Application Areas】

• Citrus orchard disease surveillance – Early detection of HLB to prevent outbreaks.

• Quarantine inspection stations – Screening plant materials and nursery stock.

• Plant pathology research – Studying HLB infection patterns, transmission, and control methods.

• Agricultural diagnostics – Supporting disease prevention and crop protection programs.

The MIRA Citrus Huanglongbing Fluorescence Detection Kit offers a fast, accurate, and efficient solution for HLB detection, aiding in sustainable citrus production and effective disease management.

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