This kit leverages Multienzyme Isothermal Rapid Amplification (MIRA) technology, operating at room temperature or a constant temperature range of 39ºC to 42ºC. By utilizing auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex that searches for and binds to the target's homologous domain. This process results in the formation of a D-loop region at the homologous site, triggering strand exchange. As the recombinase dissociates from the complex, the polymerase binds to the 3' end of the primer, initiating chain extension. MIRA technology is ideal for laboratory-based DNA amplification and other detection applications, offering a reliable and efficient solution for molecular research.
【Features】
High Sensitivity & Strong Specificity
Achieves reliable and precise DNA amplification.
Fast Reaction Time
Results in 30 minutes.
Easy to Use
The reaction components are provided in dry powder form, simplifying both operation and storage.
Low-Hassle Setup
No need for expensive PCR machines. The reaction can be performed using affordable metal or water baths, making it cost-effective for any lab.
【Primer Design】
For optimal performance, we recommend primers with a length of 30-35 bp. Short primers may reduce amplification speed and detection sensitivity. To ensure efficient amplification, primers should be designed to avoid secondary structures that could interfere with the process. The ideal amplicon length is between 150-500 bp for the best results.
【Packing Specifications】
- Specifications：48 T/pac
【Kit Composition】
|
Composition |
Content |
|
A buffer |
1.6mL×1Tube |
|
B buffer |
150μL×1Tube |
|
Positive control template |
100μL×1Tube |
|
Positive control primer mix |
60μL×1Tube |
|
Reagent |
48 T |
|
Guide Manual |
1 Copy |
【Kit Storage】
- Storage Conditions: Store at ≤-20ºC in a constant temperature environment. Keep away from light and avoid heavy pressure.
- Shelf Life: 14 months.
【Note】
- Self-designed primers/probes must be added for MIRA project development.
- The kit is available in two types - DNA and RNA. The RNA type includes reverse transcriptase, allowing amplification with RNA, dsDNA, and ssDNA as templates. The DNA type can only amplify with dsDNA as the template.
- Available in three types - Basic, Fluorescent, and Test Strip. Choose the appropriate kit based on your needs and use the corresponding primers/probes.
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