This kit with a rapid nucleic acid amplification method that operates effectively at room temperature or within a constant temperature range (typically 39℃–42℃). The process begins with reverse transcriptase using specific primers to synthesize complementary DNA from RNA templates. The reaction proceeds with the assistance of auxiliary proteins and single-strand binding proteins, allowing the recombinase and primer to form a complex. This complex searches for and binds to the target homology region, forming a D-loop and initiating strand exchange. Once recombinase dissociates, polymerase binds to the 3' end of the primer and begins chain extension. This kit is ideal for RNA amplification at the laboratory level, as well as for various other RNA-based detection applications.
【Features】
Enhanced Detection Sensitivity
Optimized for precise RNA amplification, offering superior sensitivity.
Quick Results
Complete reaction in just 30 minutes, making it highly efficient.
Convenient Handling
The dry powder format ensures ease of use and long-term storage stability.
Less Requirements
The amplification reaction can be performed using basic laboratory equipment like metal or water baths, eliminating the need for costly PCR amplifiers.
【Primer design】
Primers should ideally be 30-35 bp in length to ensure optimal amplification efficiency. Shorter primers may reduce amplification effectiveness and detection sensitivity. The primers are designed to minimize the formation of secondary structures that could hinder amplification. The recommended product amplicon size is between 150 and 500 bp.
【Kit Composition】
|
Composition |
Content |
|
A buffer |
1.6mL×1Tube |
|
B buffer |
150μL×1Tube |
|
Reagents |
48T |
|
Guide Manual |
1 Copy |
Note: Considering the problem of nucleic acid degradation, RNA series products do not provide positive control templates and primers.
【Packing Specifications】
- Specifications：48 T/pack
【Kit storage】
- Storage Conditions: Store at ≤-20ºC in a constant temperature environment. Keep away from light and avoid heavy pressure.
- Shelf Life: 14 months.
【Note】
- Self-designed primers/probes must be added for MIRA project development.
- The kit is available in two types - DNA and RNA. The RNA type includes reverse transcriptase, allowing amplification with RNA, dsDNA, and ssDNA as templates. The DNA type can only amplify with dsDNA as the template.
- Available in three types - Basic, Fluorescent, and Test Strip. Choose the appropriate kit based on your needs and use the corresponding primers/probes.
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