MIRA Neisseria Gonorrhoeae Nucleic Acid Test Strip Detection Kit Research Use Only

【Product name】 MIRA Neisseria Gonorrhoeae Nucleic Acid Test Strip Detection Kit(Research Use Only)

【Packing Specifications】48 T/pack

This kit The MIRA Neisseria Gonorrhoeae Nucleic Acid Test Strip Detection Kit is designed for the qualitative detection of Neisseria gonorrhoeae (the causative agent of gonorrhea). This kit is intended for research use only and does not involve virus typing. It uses a rapid, constant temperature isothermal nucleic acid amplification technology for sensitive and efficient detection.

At a constant temperature (typically 39ºC–42ºC), reverse transcriptase synthesizes complementary DNA (cDNA) from RNA templates using specific primers. The newly synthesized cDNA serves as a template for further amplification, assisted by recombinant enzymes, single-stranded DNA binding proteins, and DNA polymerase. During amplification, the modified specific molecular probe, along with the modified reverse primer, forms a double-ended modified nucleic acid fragment. As the amplification products accumulate, the final results are interpreted visually on nucleic acid test strips, providing clear and reliable results.

Add 37.5 μL E buffer to each freeze-dried reagent.

● Nucleic Acid Extraction: Choose an appropriate nucleic acid extraction method and reagents to extract the sample's DNA.

● Reaction Setup:

Add 10 μL of the nucleic acid template to each reaction tube (adjust the template amount with sterile water to make up a total of 10 μL).

For the positive control, add 10 μL of the positive control template and 10 μL of sterile water to the negative control.

● Buffer Addition and Mixing:

Add 2.5 μL of B buffer to each reaction tube. For multiple reactions, it is recommended to add the B buffer to the inside of the cap.

Seal the tubes and invert them 8-10 times to mix the contents thoroughly. After mixing, centrifuge briefly or flick the tubes to collect the mixture at the bottom, then transfer the tubes to the amplification area.

1. The reagent is amplified in a metal bath at 39 ~42 ℃ for 10-15 min ,the reaction time could be extended appropriately if the sample concentration is lower, but it is recommended not over 20 min.

2. Positive control: make sure the quality control line and the detection line have strips;

The above requirements of the same experiment should be met at the same time, otherwise the experiment will be deemed invalid;

4. Negative and positive controls should be set for the experiment.

3. Different brands of test strips may have different performance.

1. Storage conditions: storage temperature ≤-20ºC ,keep in dark and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for production date.

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