【Product Name】MIRA Herpes Smplex Virus Fluorescence Detection Kit(Research Use Only)
【Packing Specifications】48 T/pack
【Intended usage】This kit is suitable for general qualitative detection of Herpes Smplex Virus without virus typing.
【Detection principle】
This kit is based on a rapid nucleic acid isothermal amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands and the recombinase, Single-stranded DNA-binding proteins and DNA polymerases use newly synthesized cDNA strands as templates for rapid nucleic acid amplification reactions. With the accumulation of amplification products, the designed specific molecular probes release fluorescent signals under the action of exonuclease, and the use of fluorescence detection equipment can realize real-time monitoring of the amplification process of target fragments.
【Main components】
|
Composition |
Content |
Main ingredient |
|
E buffer |
1.0 ml *2 tubes |
Reaction buffer |
|
B buffer |
150 μL *1 tube |
Mg2+and so on |
|
Positive control template(084) |
100 μL *1 tube |
synthetic plasmid |
|
Reagents |
48T |
Enzymes, dNTPs, etc. |
【Program setting】
The FAM channel collects fluorescence signals once every 30 seconds, and sets 40 cycles in 39ºC.
Negative control Ct value ≥35 or not detected;
Positive control Ct value ≤ 20;
If conduct the same experiment,it must meet the above requirements meanwhile, otherwise the experiment will be considered invalid.
Adjust the baseline (Baseline) and threshold (Threshold) of the detection channel according to the analyzed fluorescence curve image. The baseline start is set to 1~2 cycles, the end is set to the smallest CT value cycle in this group of data (generally, it can be set to 2~2 cycles. 6), so that each parameter meets the requirements in Quality Control.
The FAM channel amplification curve increases exponentially and Ct<35 is positive;
The FAM channel amplification curve has no Ct value or Ct ≥35 is negative.
Due to differences in methodology, abnormal fluorescence curves may occur when using some brands of fluorescent PCR
【Storage】
1. Storage conditions: ≤-20ºC .
2. Validity period: 14 months;
Hot Tags: mira herpes simplex virus fluorescence detection kit research use only, China mira herpes simplex virus fluorescence detection kit research use only manufacturers, suppliers, Neisseria Gonorrhoeae Fluorescence Detection, Neisseria Gonorrhoeae Nucleic acid test strip Detection Kit, Monkeypox Virus Nucleic acid test strip Detection Kit, reproductive pathogen detection kit, Chlamydia Trachomatis Nucleic acid test strip, Herpes Simplex Virus Fluorescence Detection Kit
