MIRA Monkeypox Virus Fluorescence Detection Kit Research Use Only

In August, the World Health Organization declared that the monkeypox epidemic constituted a "public health emergency of international concern." This is the second time since July 2022 that the World Health Organization has issued the highest level alert on the monkeypox epidemic, which has attracted great attention from the international community.

Monkeypox is a viral zoonotic disease that mainly invades the human body through mucous membranes and damaged skin. It can also be spread through contact with virus-contaminated items and respiratory droplets.

Recently, the scope of the monkeypox epidemic has continued to expand. Especially during the peak tourist season and frequent international exchanges, the risk of cross-border transmission of monkeypox virus increases. Early warning and prevention of monkeypox are key.

Nucleic acid amplification detection methods are routinely used, and monkeypox virus nucleic acid can be detected in specimens such as rashes, blister fluids, scabs, oropharyngeal or nasopharyngeal secretions.

Monkeypox is included in my country's legal Class B infectious diseases for management. Because monkeypox spreads widely and has a long incubation period, rapid and effective monkeypox screening is critical. Conventional PCR methods are limited by the experimental environment and detection time, and cannot achieve rapid screening on the front line of the epidemic, reducing the efficiency of rapid response to the monkeypox epidemic.

Amp Future Biotech brings two constant-temperature nucleic acid amplification rapid test solutions, which have the advantages of accuracy, speed, and simplicity. You can go deep into the front line with a small and lightweight instrument. It only takes about 35 minutes to complete the test with simple operations. The results are accurate and efficient, very Suitable for rapid disease screening.

Option 1

Detection is performed through isothermal nucleic acid amplification technology combined with fluorescence. It can test 8 samples at a time and issue results in 35 minutes; it can also be operated through a mobile app, which is simple and fast.

Option 2

Detection is performed through isothermal nucleic acid amplification technology combined with colloidal gold test strips. The small device is flexible and portable, and can produce results in 30 minutes. The disposable consumables can be thrown away immediately, and the sealed consumables reduce aerosol pollution.

• Sensitivity

The monkeypox virus constant temperature detection solution developed based on MIRA technology has been compared with a certain brand of QPCR detection reagent on the market, as shown in the figure below:

Fluorescence data comparison

Test strip type data comparison

Based on the fluorescent and test strip-based constant-temperature detection solutions for monkeypox virus developed by MIRA, the sensitivity of detecting positive nucleic acids is consistent with the sensitivity of a certain brand of virus detection kits.

Display of specific test results

• Specificity

Two monkeypox detection overall solutions developed based on MIRA have high sensitivity and specificity, and are overall more convenient and efficient than PCR detection. They are very suitable for front-line rapid detection of epidemics and contribute to ensuring the safety of public life.

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