1. The product is used for nucleic acid detection of animal disease pathogens, and the product for zoonotic diseases is only for scientific research use;
2. The product is suitable for nucleic acid detection extracted from animal tissues, swabs, blood and other samples. The extraction method can be to extract nucleic acid using a centrifugal column kit, extract nucleic acid using a magnetic bead method, or use our company's nucleic acid rapid release agent to treat nucleic acid;
3. The amplification detection time of DNA/RNA target detection products does not exceed 20 minutes. RNA products already contain reverse transcriptase for one-step amplification detection;
4. Single-target fluorescence detection products are generally FAM fluorescence channels. For dual detection products, please refer to the instructions for detection channels. If there are special needs, it can be customized;
5. Single-target test strips are suitable for nucleic acid test strips for detecting fluorescein (FITC/6-FAM)/biotin. For dual detection products, please refer to the instructions for modification groups (can be used with our company's dual nucleic acid test strips). If there are special needs, it can be customized;
6. The conventional product form is an in-situ freeze-dried powder freeze-dried in 8-tube PCR tubes. If there are special needs, it can be customized.
【Product Name】MIRA Yersinia Pestis (Caf1 gene) Fluorescence Detection Kit (Research Use Only)
【Packing Specifications】48 T/pack
【Background】
Yersinia pestis is the causative agent of plague, a highly virulent bacterial pathogen with significant historical and public health implications. The Caf1 gene encodes a critical virulence factor involved in bacterial pathogenesis, making it a key target for rapid and accurate detection. Early identification of Y. pestis is essential for disease surveillance, biodefense research, and epidemiological studies.
【Intended usage】
The MIRA Yersinia Pestis (Caf1 Gene) Fluorescence Detection Kit is designed for the rapid and sensitive detection of Y. pestis through nucleic acid amplification. Utilizing MIRA (Multienzyme Isothermal Rapid Amplification) technology, this assay enables fast and highly specific detection at a constant temperature (39–42°C) without requiring thermal cycling. It is intended for research use only and is ideal for applications in biodefense, epidemiological surveillance, and infectious disease research.
【Main components】
|
Composition |
Content |
Main ingredient |
|
E buffer |
1.0 ml *2 tubes |
Reaction buffer |
|
B buffer |
150 μL *1 tube |
Mg2+and so on |
|
Positive control template(084) |
100 μL *1 tube |
synthetic plasmid |
|
Reagents |
48T |
Enzymes, dNTPs, etc. |
【Key Features】
Rapid and efficient detection – Results available in 15–20 minutes, enabling timely pathogen identification.
Real-time fluorescence monitoring – Ensures accurate and quantitative detection. Minimized contamination risk – Conducts amplification at a constant temperature, reducing aerosol contamination concerns.
Freeze-dried reagents – Stable at room temperature, easy to transport, and ideal for POCT applications.
【Application Areas】
• Biodefense and biosecurity – Rapid detection of Y. pestis for emergency response.
• Epidemiological research – Monitoring outbreaks and transmission patterns.
• Microbiological studies – Understanding Y. pestis virulence mechanisms.
The MIRA Yersinia Pestis (Caf1 Gene) Fluorescence Detection Kit provides a fast, portable, and highly sensitive solution for on-site and laboratory detection, supporting research in pathogen surveillance and infectious disease control.
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