MIRA Yersinia Pestis Pla/caf1 Gene Nucleic Acid Test Strip Detection Kit Research Use Only

1. The product is used for nucleic acid detection of animal disease pathogens, and the product for zoonotic diseases is only for scientific research use;

2. The product is suitable for nucleic acid detection extracted from animal tissues, swabs, blood and other samples. The extraction method can be to extract nucleic acid using a centrifugal column kit, extract nucleic acid using a magnetic bead method, or use our company's nucleic acid rapid release agent to treat nucleic acid;

3. The amplification detection time of DNA/RNA target detection products does not exceed 20 minutes. RNA products already contain reverse transcriptase for one-step amplification detection;

4. Single-target fluorescence detection products are generally FAM fluorescence channels. For dual detection products, please refer to the instructions for detection channels. If there are special needs, it can be customized;

5. Single-target test strips are suitable for nucleic acid test strips for detecting fluorescein (FITC/6-FAM)/biotin. For dual detection products, please refer to the instructions for modification groups (can be used with our company's dual nucleic acid test strips). If there are special needs, it can be customized;

6. The conventional product form is an in-situ freeze-dried powder freeze-dried in 8-tube PCR tubes. If there are special needs, it can be customized.

【Product name】 MIRA Yersinia Pestis(Pla gene) Nucleic Acid Test Strip Detection Kit(Research Use Only)

【Packing Specifications】48 T/pack

【Intended usage】This kit is for research use only.This kit is suitable for the qualitative detection of Yersinia Pestis(Pla gene) without virus typing

This kit is based on a constant temperature isothermal nucleic acid rapid amplification technology: at constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA chain, and the recombinant enzyme, single strand DNA binding protein and DNA polymerase in the reaction system use the newly synthesized cDNA chain as template for rapid nucleic acid amplification reaction. With the help of endonuclidenase, the modified specific molecular probe is amplified with the modified reverse primer to form a double-ended modified nucleic acid fragment. With the accumulation of amplified products, nucleic acid test strips can be used for color interpretation of the final result.

The number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.

Sample processing(processing area)

Nucleic acid extraction

Select the appropriate nucleic acid extraction method and reagents to extract.

Add 10 μL of nucleic acid template to the reaction tube (the amount of template can be adjusted and make up with sterile water; that is, nucleic acid template plus sterile water is 10 μL in total), add 10μL of positive control template to the positive control, and add 10 μL of sterile water;

Add 2.5μL B buffer to each reaction tube, and cover the cap (for multiple reactions, it is recommended to add the B buffer to the inside of the cap of the reaction tube); invert the reaction tube 8-10 times to mix well, after mixing, put The reaction solution is swished (or quickly centrifuged) to the bottom of the tube and transferred to the amplification area.

Nucleic acid amplification (amplification area)

The reagent was amplified in a metal bath at 39 ~42 ℃ for 10-15 min (the reaction time could be extended appropriately if the sample concentration was lower, but it is recommended not to exceed 20 min).

Result display

1. 5 minutes.(The probe in this kit is FAM modified, and the downstream primer is Biotin modified. Please select an appropriate nucleic acid test strip for detection. Our company's nucleic acid testline has strips, and the detection line has no strips;

2. Positive control: both the quality control line and the detection line have strips;

3. The above requirements of the same experiment should be met at the same time, otherwise the experiment will be deemed invalid;

4. Negative and positive controls should be set for the experiment.

5. Different brands of test strips may have different performance.

1. Please read the instructions carefully before use and strictly follow the operation steps;

2. Sample handling should be conducted in strict accordance with biosafety specifications. During the experiment, work clothes and disposable gloves should be worn, and the experiment should be conducted in different areas to avoid cross-contamination (nucleic acid test strips should be opened for color development, and pollution should be strictly prevented);

3. Use the reagent within the validity period, and the components shall not be mixed with

1. Storage conditions: storage temperature ≤-20ºC constant temperature environment,keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for production date.

Manufacturer:AMP-Future(Changzhou) Biotechnology Co., Ltd.

Address: Floor 4, Building 9#, Innovation Park, Changzhou, Israel, China, No. 18-67, Changwu Middle Road, Wujin District, Changzhou City, Jiangsu Province

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