MIRA Newcastle Disease Virus Fluorescence Detection Kit

1. The product is used for nucleic acid detection of animal disease pathogens such as poultry, dogs and cats;

2. The product is suitable for nucleic acid detection extracted from animal tissues, swabs, blood and other samples. The extraction method can be to extract nucleic acid using a centrifugal column kit, extract nucleic acid using a magnetic bead method, or use our company's nucleic acid rapid release agent to treat nucleic acid;

3. The amplification detection time of DNA/RNA target detection products does not exceed 20 minutes. RNA products already contain reverse transcriptase for one-step amplification detection;

4. Single-target fluorescence detection products are generally FAM fluorescence channels. For dual detection product detection channels, please refer to the instructions. If there are special needs, they can be customized;

5. Single-target test strip type detection products are suitable for nucleic acid test strips for detecting fluorescein (FITC/6-FAM)/biotin. If there are special needs, they can be customized;

6. The conventional product form is in situ freeze-dried powder and freeze-dried in 8-tube PCR tubes. If there are special needs, they can be customized.

【Product Name】MIRA Newcastle Disease Virus Fluorescence Detection Kit

【Packing Specifications】48 T/pack

【Intended usage】This kit is for research use only.This kit is suitable for general qualitative detection of Newcastle Disease Virus without virus typing.

This kit is based on a rapid nucleic acid isothermal amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands and the recombinase, Single-stranded DNA-binding proteins and DNA polymerases use newly synthesized cDNA strands as templates for rapid nucleic acid amplification reactions. With the accumulation of amplification products, the designed specific molecular probes release fluorescent signals under the action of exonuclease, and the use of fluorescence detection equipment can realize real-time monitoring of the amplification process of target fragments.

Storage conditions: storage temperature ≤-20ºC constant temperature environment, keep away from light and avoid heavySelect the appropriate nucleic acid extraction method and reagents to extract sample nucleic acid;

Add 10 μL of nucleic acid template to the reaction tube (the amount of template can be adjusted and make up with sterile water; that is, nucleic acid template plus sterile water is 10 μL in total), add 10μL of positive control template to the positive control, and add 10 μL of sterile water;

 Add 2.5μL B buffer to each reaction tube, and cover the cap (for multiple reactions, it is recommended to add the B buffer to the inside of the cap of the reaction tube); invert the reaction tube 8-10 times to mix well, after mixing, put The reaction solution is swished (or quickly centrifuged) to the bottom of the tube and transferred to the amplification area.

Nucleic acid amplification (amplification area)

Different program setting methods can be selected according to different application requirements:

Program setting scheme two

Program settings

At 39ºC,the FAM channel collects fluorescence signals once every 30 seconds, and sets 40 cycles.

Quality Control

Negative control Ct value ≥35 or not detected; positive control Ct value ≤ 20; the same experiment must meet the above requirements at the same time, otherwise the experiment will be considered invalid.

Adjust the baseline (Baseline) and threshold (Threshold) of the detection channel according to the analyzed fluorescence curve image. The baseline start is set to 1~2 cycles, and the end is set to the smallest CT value cycle in this group of data (generally, it can be set to 2~2 cycles. 6), so that each parameter meets the requirements in "3.2.2 Quality Control", and then judge whether it is negative or positive: the FAM channel amplification curve increases exponentially and Ct<35 is positive; the FAM channel amplification curve has no Ct value or Ct ≥35 is negative.

Due to differences in methodology, abnormal fluorescence curves may occur when using some brands of fluorescent PCR instruments on the market. It is recommended to use our equipment or Applied Biosystems series fluorescent quantitative PCR pressure;

Product validity period: 14 months;

See the outer packaging for the production date.

Manufacturer:AMP-Future(Changzhou) Biotechnology Co., Ltd.

Address: Floor 4, Building 9#, Innovation Park, Changzhou, Israel, China, No. 18-67, Changwu Middle Road, Wujin District, Changzhou City, Jiangsu Province

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