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MIRA Influenza B Virus Nucleic Acid Test Strip Detection Kit Research Use Only

MIRA Influenza B Virus Nucleic Acid Test Strip Detection Kit Research Use Only

This kit is based on a constant temperature isothermal nucleic acid rapid amplification technology: at constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA chain, and the recombinant enzyme, single strand DNA binding protein and DNA polymerase in the reaction system use the newly synthesized cDNA chain as template for rapid nucleic acid amplification reaction.

Product Introduction

1. The product is only for scientific research and is used for nucleic acid detection of respiratory pathogens;

2. The product is suitable for nucleic acid detection extracted from samples such as throat and nasal swabs. The extraction method can be to extract nucleic acid using centrifugal column kit, magnetic bead method, or use our company's nucleic acid rapid release agent;

3. The amplification detection time of DNA/RNA target detection products does not exceed 20 minutes. RNA products already contain reverse transcriptase for one-step amplification detection;

4. Single-target test strip type detection products are suitable for nucleic acid test strips for detecting fluorescein (FITC/6-FAM)/biotin (Biotin). If there are special needs, they can be customized;

5. The conventional product form is an in-situ freeze-dried powder freeze-dried in an 8-piece PCR tube. If there are special needs, it can be customized.

 

Product name MIRA Influenza B Virus Nucleic Acid Test Strip Detection Kit (Research Use Only)

Packing Specifications48 T/pack

Intended usageThis kit is for research use only.This kit is suitable for the qualitative detection of Influenza B,without virus typing

 

Detection principle

 

 

This kit is based on a constant temperature isothermal nucleic acid rapid amplification technology: at constant temperature (generally 39 ºC~42 ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA chain, and the recombinant enzyme, single strand DNA binding protein and DNA polymerase in the reaction system use the newly synthesized cDNA chain as template for rapid nucleic acid amplification reaction. With the help of endonuclidenase, the modified specific molecular probe is amplified with the modified reverse primer to form a double-ended modified nucleic acid fragment. With the accumulation of amplified products, nucleic acid test strips can be used for color interpretation of the final result.

 

Main components

 

 

Composition

Content

Main ingredient

E buffer

1.0 ml *2 tubes

Reaction buffer

B buffer

150 μL *1tube

Mg2+and so on

Positive control template(003)

100 μL *1tube

synthetic plasmid

Reagents

48T

Enzymes, dNTPs, etc.

 

Storage conditions and expiration date

 

 

1. Storage conditions: storage temperature≤-20ºC constant temperature environment,keep away from light and avoid heavy pressure;

2. Product validity period: 14 months;

3. See the outer packaging for production date.

 

Applicable instrument

 

 

Suitable for constant temperature control equipment such as constant temperature metal bath.

 

Sample requirement

 

 

1. Collect samples in accordance with relevant standards;

2. It is recommended to test as soon as possible after the sample is collected. The test sample can be temporarily stored at 4ºC within 24 hours; for long-term storage, it should be kept below -70ºC, and repeated freezing and thawing should be avoided;

3. If you use sample preservation solution or quick release agent to treat the sample, pay attention to the influence of the residues of substances that affect protein activity, such as acid, alkali, salt, surfactant, etc., on the performance of the reagent.

 

Interpretation of test results

 

 

1. Improper sample collection, transportation and preservation, and improper reagent transportation, preservation and preparation may affect the experimental results and even lead to false negative results;

2. If laboratory contamination, reagent contamination, sample cross-contamination, false positive results may occur;

3. Different brands of test strips may have different performance.

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