MIRA Mycoplasma Columbinum Fluorescence Detection Kit

MIRA Mycoplasma Columbinum Fluorescence Detection Kit

This kit is based on a rapid nucleic acid isothermal amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands and the recombinase, Single-stranded DNA-binding proteins and DNA polymerases use newly synthesized cDNA strands as templates for rapid nucleic acid amplification reactions.

Product Introduction

Product Name】MIRA Mycoplasma columbinum Fluorescence Detection Kit

Packing Specifications48 T/pack

 

【Background】

 

 

Mycoplasma columbinum is a significant pathogen affecting pigeons and other birds, often leading to respiratory infections and reduced flock health. Quick and accurate detection of M. columbinum is essential for avian disease control, veterinary research, and improving animal health management strategies.

 

【Intended usage】

 

 

The MIRA Mycoplasma columbinum Fluorescence Detection Kit is a rapid nucleic acid amplification assay designed for the qualitative detection of M. columbinum. Utilizing MIRA (Multienzyme Isothermal Rapid Amplification) technology, this kit allows for fast, highly specific, and sensitive detection at a constant temperature (39–42°C) without requiring traditional PCR thermal cyclers. It is intended for research use only and is ideal for applications in avian disease surveillance, veterinary diagnostics, and microbiological research.Detection principle

This kit is based on a rapid nucleic acid isothermal amplification technology: at room temperature and constant temperature (generally 39ºC~42ºC), reverse transcriptase uses specific primers and template RNA to synthesize cDNA strands and the recombinase, Single-stranded DNA-binding proteins and DNA polymerases use newly synthesized cDNA strands as templates for rapid nucleic acid amplification reactions. With the accumulation of amplification products, the designed specific molecular probes release fluorescent signals under the action of exonuclease, and the use of fluorescence detection equipment can realize real-time monitoring of the amplification process of target fragments.

 

Main components

 

 

Composition

Content

Main ingredient

E buffer

1.0 ml *2 tubes

Reaction buffer

B buffer

150 μL *1 tube

Mg2+and so on

Positive control template(084)

100 μL *1 tube

synthetic plasmid

Reagents

48T

Enzymes, dNTPs, etc.

 

【Key Features】

 

 

 Rapid results – Detection completed within 15–20 minutes, enabling timely interventions.
 Isothermal amplification – No need for complex thermal cycling equipment, simplifying the workflow.
 High specificity and sensitivity – Accurate identification of M. columbinum with minimal cross-reactivity.
 Real-time fluorescence monitoring – Ensures precise observation of amplification results.
 Lyophilized reagents – Stable at room temperature, easy to transport and store.
 User-friendly operation – Minimal technical expertise required, suitable for field and laboratory use.

 

【Application Areas】

 

 

• Veterinary diagnostics – Rapid screening for Mycoplasma columbinum in pigeon flocks.

• Avian disease research – Studying infection patterns and epidemiology.

• Epidemiological surveillance – Monitoring the spread and control of M. columbinum in bird populations.

 

The MIRA Mycoplasma columbinum Fluorescence Detection Kit delivers a fast, portable, and reliable solution for the detection of M. columbinum, supporting effective avian health management and research efforts.

 

 

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