Livestock Disease Kit with African swine fever virus Test Kit for Nucleic Acid Detection
【Product parameters】
|
Kit composition (WLRE8208KIT 48T/Pack) |
|
|
Composition |
Content |
|
E buffer |
1 mL×2 Tubes |
|
B buffer |
150 μL×1Tube |
|
Positive control template |
100 μL×1Tube |
|
Reagents |
48T |
【Product features and advantages】
|
Features |
Advantages |
|
High sensitivity |
1 copies/ml |
|
Strong specificity |
Specificity is superior to PCR |
|
Short reaction time |
20min |
|
Polymorphic reagent |
Liquid, freeze-dried powder, freeze-dried ball |
|
Easy to operate |
Few steps of liquid dispensing; It is even possible to add only samples and amplify to get the results Design of primers and probe is simple |
|
Easy to store and transport |
It is best preserved at -20℃, and can be stored at room temperature for up to 30 days in a cool place away from light. The freeze-dried form has a long storage time and is convenient for transportation |
|
Low requirements on equipment |
Applicable to Applied Biosystems 7500, QuantStudio 3/5, QuantStudio 6/7 Flex fluorescence quantitative PCR instrument; Applicable to our WL-16-III and other isothermal fluorescence detector |
【Product application】
|
Application |
|
|
Ultra-clean laboratory |
African Swine Fever Virus Isothermal Detection |
|
Indoor |
|
【Operation procedure and process】
Take out the liquid components of the kit in advance, thaw at room temperature, and shake to mix.
Prepare freeze-dried reagents according to the number of samples to be tested, negative and positive controls, and add 37.5 μL E buffer to each freeze-dried reagent.
Select the appropriate nucleic acid extraction method and reagent to extract the sample nucleic acid
Add 10 μL nucleic acid template to the reaction tube (the amount of template can be adjusted to be filled with sterile water; That is, 10 μL nucleic acid template plus sterile water), 10 μL positive control template was added to positive control, and 10 μL sterile water was added to negative control
Add 2.5μL B buffer to each reaction tube and close the tube cap (for multiple reactions, it is recommended to add B buffer to the inside of the tube cap)
Thoroughly mix the reaction tube upside and down for 8-10 times. After mixing, shake (or rapidly centrifuge) the reaction liquid to the bottom of the tube and transfer it to the amplification zone.
【Performence Comparison Data】
|
No. |
TT value |
Template concentration |
Result determination |
|
1 |
4.2 |
10-1 |
Positive |
|
2 |
5.5 |
10-2 |
Positive |
|
3 |
10.2 |
10-3 |
Positive |
|
4 |
17.0 |
10-4 |
Positive |
|
5 |
12.0 |
10-5 |
Positive |
|
6 |
- |
10-6 |
Negative |
|
7 |
- |
10-7 |
Negative |
|
8 |
Negative control |
Negative |
|
【Support and Services】
Livestock Disease Kit Technical Support and Services
We provide technical support and services for our Livestock Disease Kit. We are committed to helping you get the most out of your product and ensuring your satisfaction with our products and services.
- Our technical support staff is available to answer any questions you may have about the product.
- We offer online tutorials and user guides to help you understand the product better.
- We provide technical assistance to help troubleshoot any issues you may be having with the product.
- We offer a warranty on our products.
If you have any questions or need technical support, please do not hesitate to contact us. We are here to help you make the most of your Livestock Disease Kit.
【FAQ】
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