Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a highly pathogenic strain of E. coli that can cause severe foodborne illness, including hemorrhagic colitis and hemolytic uremic syndrome (HUS). Rapid and accurate detection of this pathogen is crucial for food safety, public health monitoring, and microbiological research.
【Intended Use】
The MIRA Enterohemorrhagic E. coli O157:H7 Fluorescence Detection Kit is a rapid, isothermal nucleic acid amplification assay designed for the qualitative detection of E. coli O157:H7. This kit utilizes MIRA (Multienzyme Isothermal Rapid Amplification) technology, enabling fast, highly specific, and sensitive pathogen identification without the need for traditional PCR equipment. It is intended for research use only and is ideal for applications in food safety testing, microbiological studies, and pathogen surveillance.
【Key Features】
- Rapid detection – Results available in 15–20 minutes, significantly faster than conventional methods.
- High sensitivity and specificity – Ensures accurate detection with minimal cross-reactivity.
- Real-time fluorescence monitoring – Enables precise tracking of amplification.
【Application Areas】
- Food safety and quality control – Detection of E. coli O157:H7 in food samples.
- Microbiological research – Pathogen detection in environmental and clinical studies.
- Public health surveillance – Monitoring outbreaks and preventing contamination.
The MIRA Enterohemorrhagic E. coli O157:H7 Fluorescence Detection Kit provides a fast, reliable, and efficient solution for detecting this dangerous pathogen, supporting efforts to enhance food safety and disease prevention.
【Main components】
|
Composition |
Content |
Main ingredient |
|
E buffer |
1.0 ml *2tubes |
Reaction buffer |
|
B buffer |
150 μL *1tube |
Mg2+and so on |
|
Positive control template(016) |
100 μL *1tube |
synthetic plasmid |
|
Reagents |
48T |
Enzymes, dNTPs, etc. |
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