Dual Detection of Genetically Modified Crops Using CRISPR Technology and Cas12a/Cas13a
- Paper: Development of a novel Cas13a/Cas12a-mediated 'one-pot'dual detection assay for genetically modified crops
- Journal: Journal of Advanced Research
- Impact Factor: 11.4
- Product Name: DNA lsothermal Rapid Amplification Kit (basic)
- Catalog No: WLB8201KIT
| Project | Method | Fluorescent Probe Labeling | Test Strip Probe Labeling | Time | Sensitivity |
| Detection of CaMV35S and NOS Genes in Genetically Modified Crops |
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35 min | 20copies/test |
MIRA Combed with CRISPR
- Study on One-Tube Method Combining CRISPR Technology, Isothermal Amplification, and Cas12a. Article：Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a
- Paper：Nature Biomedical Engineering
- Impact Factor：29.2
- Product Name：DNA lsothermal Rapid Amplification Kit (basic)
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Project |
Method |
Time |
Sensitivity |
Performance |
Note |
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Novel Coronavirus (COVID-19) - Orf1ab Gene, N Gene, E Gene HPV18 HCMV (Human Cytomegalovirus)
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One-Step Detection Using MIRA Isothermal Amplification and Cas12a Cleavage System Investigating the impact of the suboptimal PAM region on the competitive relationship between nucleic acid amplification and the Cas cleavage system in one-step detection. This method was validated across different pathogens and genes, ultimately establishing the practicality of the sPAMC detection method. |
20min |
1copy/μL |
Sensitivity: 94.2% Specificity: 100%
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Compared the performance differences between Amp Future reagents and other reagents. Amp reagents are better suited for the Cas system. |
