Collaboration Case with Customs Authorities
Establishment of Multiple Nucleic Acid Detection Methods for Avian Hepatitis E Virus
Background: There is a high demand for nucleic acid testing by China's customs entry and exit quarantine departments.
Due to the specific nature of the customs system, only methods outlined in national or customs industry standards can be used for testing and issuing results.
Current Situation:
At present, the nucleic acid testing methods used by the customs system are generally conventional PCR, nested PCR, and fluorescence quantitative PCR.
PCR technology relies on large equipment, and samples collected at ports must be sent to standard laboratories for testing, which is cumbersome and time-consuming.
Demand:
MIRA technology, ideal for on-site rapid testing, has gained recognition from customs clients. To meet the demand for animal entry and exit quarantine, we are collaborating to develop MIRA test kits.
We provide technical support to help establish industry standards and promote MIRA's application in the customs system.
| Amplification Technology |
MIRA |
RT-qPCR |
Nested PCR |
|
Temperature Setting |
42℃ |
50℃-95℃-95℃-60℃ |
FirstRT-PCRAmplification: 50℃-95℃-95℃-55℃-72℃ Second PCR Amplification: 95℃-60℃-72℃ |
|
Equipment Required |
Isothermal fluorescence detection device or qPCR instrument |
qPCR instrument |
PCR instrument, electrophoresis system, gel imaging system |
|
Amplification Time |
20min |
90min |
200 min |
|
Standard Sensitivity |
20 copies/μL |
20 copies/μL |
0.8 copies/μL |
|
Cost-Effectiveness |
★★★★★ |
★★★ |
★ |
No1：2500copies/μL No2：500copies/μL
No3：100copies/μL No4：20copies/μL
No5：4copies/μL No6：0.8copies/μL